São Paulo is the eleventh map available in Mini Metro, being unlocked by delivering 500 passengers on San Francisco or Montréal. It is based around where São Paulo is located in the real world. Delivering 500 passengers on São Paulo unlocks Seoul, and the Bilhete Único (Universal Ticket) achievement.

The map is unique because new stations get added relatively slowly, in contrast to Osaka or Hong Kong. However, the stations that do exist have a relatively high flow of passengers. This makes the map harder and clusters of the same station type difficult to deal with. It is advised that one plans the network layout with care.

Delivering 1200 passengers using only loops unlocks the São Paulo Grand Prix achievement and the Extreme version of the map.